WebApr 22, 2024 · To examine whether LIMD1 could undergo phase separation in cells, we built upon a previously described “OptoDroplets” system in which light-sensitive chimeric proteins are assembled from protein of interest combined with the light-sensitive oligomerization domain of Arabidopsis thaliana cryptochrome (CRY2) photolyase homology region WebSep 22, 2024 · These diverse liquid-like droplets of biomolecules, which self-assemble within another liquid—the cytoplasm or nucleoplasm—are increasingly recognized as arising from a physicochemical process known as liquid-liquid phase …
Spatiotemporal regulation of insulin signaling by liquid ... - Nature
WebJul 1, 2024 · Fusing optoDroplets are highlighted by green arrowheads and in the magnified insets. ... and the protein concentration was determined by Bradford assay. In vitro 53BP1 condensation. A mix of 53BP1 W1495A (1203–1972) and mCherry-53BP1 W1495A (1203–1972; 9:1 ratio) was diluted with Ficoll 400 (Sigma-Aldrich) to a final concentration … WebJan 1, 2024 · The formation and dissociation of these optodroplets can be followed over time in living cells by fluorescence microscopy to evaluate the propensity of proteins to demix and to form phase-separated subcompartments. The optodroplet assay is based on the fusion of a protein of interest with the photolyase homology region (PHR) protein … chinese and the transcontinental railroad
An Optogenetic Toolkit for the Control of Phase Separation
WebJan 5, 2024 · The optoDroplets technique is based on optogenetics, or using light to manipulate proteins. Researchers inserted in human and mouse cells a gene from the mouse-ear cress ( Arabidopsis thaliana ), a plant similar to cabbage and mustard. The gene encodes a light-sensitive protein. WebJan 4, 2024 · OptoDroplet relies on a technique called optogenetics, involving proteins whose behavior can be altered by exposure to light. (Cells are mostly water and thus … WebDec 5, 2024 · Several assays and standards are recommended to determine whether the proteins undergo LLPS, including assessing the turbidity of the solution, microscopic examination of liquid droplets, monitoring the … chinese and western family values